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(D) The ratio of Ex4a(+)WT1 to 17AA(+)WT1 isoforms

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(D) The ratio of Ex4a(+)WT1 to 17AA(+)WT1 isoforms was firm by RT CR using Ex4-F and Ex6-R primer pair that amplifies the two Ex4a(+)WT1 and main WT1 isoforms in two unique WT1-expressing most cancers cells (LU99B and K562). (B-D) Actin is made use of as an internal management. Success are consultant of a few independent experiments.(D) The ratio of Ex4a(+)WT1 to 17AA(+)WT1 isoforms was determined