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D from Jackson Laboratories. Experimental protocols had been authorized by WEHI's

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D from Jackson Laboratories. Experimental protocols had been authorized by WEHI's Animal Ethics Committee. AML generation. MSCV retroviruses encoding internal ribosomal entry internet site (IRES)/GFP, MLL-AF9/IRES/GFP39 or MLL-ENL/IRES/GFP DNA58 were ready by transfection of Phoenix cells.59 For infection, cells prepared from 3 cryopreserved fetal livers of every genotype in stem cell medium (SCM;