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(D) The ratio of Ex4a(+)WT1 to 17AA(+)WT1 isoforms was determined by RT CR working with Ex4-F and Ex6-R primer pair that amplifies each Ex4a(+)WT1 and main WT1 isoforms in two unique WT1-expressing cancer cells (LU99B and K562). (B-D) Actin is applied as an inside control. Effects are agent of 3 unbiased experiments.(D) The ratio of Ex4a(+)WT1 to 17AA(+)WT1 isoforms was firm by RT CR making use of
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